Madrasin,≥98%

产品属性 Product Attributes

基本信息

CAS 374913-63-0
英文名称: Madrasin
别名： DDD00107587

分子式: C16H17N5O2

分子量: 311.34
规格: 10mg ; 5mg ; 25mg ; 50mg ; 100mg
溶解性： Soluble in DMSO
纯度： ≥98%
外观(性状): Light yellow to yellow Solid
储存条件: Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
运输条件: 冷藏运输

生物活性 以下数据均来自公开文献, ACMEC暂未进行独立验证, 仅供参考。

靶点 DNA/RNA Synthesis

通路 Cell Cycle;DNA Damage/DNA Repair
背景说明:

Madrasin是一个很强的细胞渗透拼接抑制剂,能够扰乱剪接体组装的前期过程。

生物活性

Madrasin (DDD00107587) is a splicing inhibitor that prevents formation of both splicing intermediates and products in vitro and interferes with one or more early steps in the pathway of spliceosome assembly. Madrasin also can inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells[1].

In Vitro

Madrasin (10-30 μM; 4-24 hours; HeLa cells) treatment inhibits the splicing of each of the RIOK3， BRD2， and Hsp40， MCL1， CCNA2， AURKA and p27 pre-mRNAs in both HeLa and HEK293 cells[1].Madrasin (10-30 μM; 4-24 hours; HeLa and HEK293 cells) treatment shows a dose- and time-dependent inhibitory effect on cell cycle progression[1].RT-PCR[1]：Cell Line:HeLa cells；Concentration:10 μM， 20 μM， or 30 μM；Incubation Time: 4 hours， 8 hours， or 24 hours；Result:Increased in inhibition of pre-mRNA splicing.Cell Cycle Analysis[1]：Cell Line:HeLa and HEK293 cells；Concentration:10 μM， 20 μM， or 30 μM;Incubation Time:4 hours， 8 hours， or 24 hours;Result:In the presence of 10 μM 8 hours after treatment， the proportion of cells in G2， M， and S phases increased， with a concomitant decrease in the number of G1 phase cells. This effect increased over time， with >40% of cells in G2 and M phase and >50% in S phase 24 hours.

数据来源文献

[1]. Andrea Pawellek et al. Identification of Small Molecule Inhibitors of Pre-mRNA Splicing. J Biol Chem， 2014 Dec 12， 289(50): 34683-34698.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用，-20°C储存时，请在6个月内使用。

产品介绍 Product Introduction

Madrasin是一个很强的细胞渗透拼接抑制剂，能够扰乱剪接体组装的前期过程。

注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。