Recombinant Human TNF/TNFa/TNFSF2(C-polyHis),BR

产品属性 Product Attributes

基本信息

别名： Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;TNF;TNFA;TNFSF2
规格: 10ug ; 50ug ; 500ug ; 1mg
外观(性状)： Lyophilized from a 0.2 μm filtered solution of 20mM PB，150mM NaCl,pH7.4.
纯度: ≥95% as determined by reducing SDS-PAGE.
储存条件: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输条件： The product is shipped at ambient temperature.

产品信息

背景说明： Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages，monocytes，neutrophils，T-cells，and NK-cells following stimulation by bacterial LPS.Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α.Synthesis of TNF-α can be induced by many different stimuli including interferons，IL2，and GM-CSF.The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever，dose-limiting hypotension，hepatotoxicity，intravascular thrombosis，and hemorrhage.Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest.Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7，exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α，which binds to both murine TNF receptors.Based on these results，many TNF-α mutants that selectively bind to TNF-R55 have been designed.These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo.Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8，Lys9，Arg10 and Phe157.This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
来源： E.coli

其它标识

溶剂: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
内毒素: Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test.

产品介绍 Product Introduction

Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus.

注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。