人基底细胞粘附分子检测试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 人基底细胞粘附分子检测试剂盒
英文名称： Human BCAM ELISA Kit
别名： AU; CD239; LU; MSK19; basal cell adhesion molecule (Lutheran blood group)
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： BCAM
样品类型： 血清/血浆/细胞培养上清
反应性： Human
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 54.68-3500 pg/ml
灵敏度： 27.35 pg/ml
特异性： 不与人BCAM类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Elisa信息

Swiss Prot： P50895
Gene ID： 4059
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人 BCAM单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人 BCAM会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人 BCAM抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人 BCAM发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人 BCAM，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人 BCAM浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人 BCAM的浓度。
背景说明： The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

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