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人白细胞介素18受体1检测试剂盒,BR
Human IL-18R1/CD218a ELISA KIT
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产品属性 Product Attributes
基本信息
中文名称: 人白细胞介素18受体1检测试剂盒英文名称: Human IL-18R1 ELISA Kit
别名: CDw218a; IL18R1; IL18RA; IL-18Ralpha; IL-18R-alpha; IL18Ralpha2; IL1RRP; IL-1Rrp; Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: IL18R1
样品类型: 血清/血浆/细胞培养上清
反应性: Human
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 7.8-500pg/mL
灵敏度: 4pg/mL
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
Elisa信息
Swiss Prot: Q13478Gene ID: 8809
检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IL-18R1 / CD218a单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人IL-18R1 / CD218a会与酶标板上的包被抗体充分结合;洗板后加入HRP酶标记的抗人IL-18R1 / CD218a抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人IL-18R1 / CD218a发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人IL-18R1 / CD218a,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人IL-18R1 / CD218a浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人IL-18R1 / CD218a的浓度。
背景说明: Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
产品介绍 Product Introduction
暂无数据注意事项 Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。