小鼠血小板衍生生长因子AAElisa试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 小鼠血小板衍生生长因子AA检测试剂盒
英文名称： Mouse PDGF-AA ELISA Kit
别名： PDGF1; PDGF-A; PDGFAA; Platelet-derived Growth Factor AA
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： PDGF-AA
样品类型： 血清/血浆/细胞培养上清
反应性： Mouse
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 25-1600pg/mL
灵敏度： 4pg/mL
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%

Elisa信息

Swiss Prot： P20033
Gene ID： 15890
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠PDGF-AA单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠PDGF-AA会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠PDGF-AA抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠PDGF-AA发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠PDGF-AA，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠PDGF-AA浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠PDGF-AA的浓度。
背景说明： Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

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注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。