小鼠骨形态发生蛋白6检测试剂盒,BR

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基本信息

中文名称： 小鼠骨形态发生蛋白6检测试剂盒
英文名称： Mouse BMP6 ELISA Kit
别名： BMP6; BMP-6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： BMP6
样品类型： 血清/血浆/细胞培养上清
反应性： Mouse
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 125-8000 pg/ml
灵敏度： 62.5 pg/ml
特异性： 不与小鼠ApoAI，BMP1，BMP2，BMP3，BMP4，HGF，HSP27，IFN，IL-1β，IL-1RA，IL-2，IL-5，IL-6，IL8，IL-10，IL-12，IL-13，IL-15，IL-17C，IL-23，MMP-2，MMP-9，sIL-2R，sIL-6R，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TNF-α，TNFRI，TNFRII，VEGF反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3.5h

Elisa信息

Swiss Prot： P20722
Gene ID： 12161
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠BMP-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠BMP-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠BMP-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠BMP-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠BMP-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠BMP-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠BMP-6的浓度。
背景说明： Bone morphogenetic protein 6 (BMP6) is a protein that in humans is encoded by the BMP6 gene. [1][2] The protein encoded by this gene is a member of the TGFβ superfamily. Bone morphogenetic proteins (BMPs) are known for their ability to induce the growth of bone and cartilage. BMP6 is able to induce all osteogenic markers in mesenchymal stem cells. The BMPs are a family of secreted signaling molecules that can induce ectopic bone growth. BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. As of April 2009, an additional function of BMP6 has been identified as described in Nature Genetics April; 41 [4]:386-8. BMP6 is the key regulator of hepcidin, the small peptide secreted by the liver which is the major regulator of iron metabolism in mammals. BMP6 is expressed in normal and atherosclerotic smooth muscle cells,[3] normal and malignant prostatic tissue,[4] healthy and osteoarthritic human articular chondrocytes and can stimulate matrix synthesis in vitro.[5] Bone morphogenetic proteins 2 and 6, expressed in arthritic synovium, are regulated by proinflammatory cytokines and differentially modulate fibroblast-like synoviocyte apoptosis.[6]

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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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