猴免疫球蛋白E检测试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 猴免疫球蛋白E检测试剂盒
英文名称： Monkey IgE ELISA Kit
别名： IgE; Immunoglobulin E
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： IgE
样品类型： 血清/血浆/细胞培养上清
反应性： Monkey
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 0.78-50ng
灵敏度： 0.39ng/mL
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%

Elisa信息

检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猴 IgE单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的猴 IgE会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗猴 IgE抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的猴 IgE发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的猴 IgE，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的猴 IgE浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中猴 IgE的浓度。
背景说明： Immunoglobulin E (IgE) is a kind of antibody (or immunoglobulin (Ig) isotype) that has only been found in mammals. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgE's main function is immunity to parasites such as helminths[1] like Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica.[2] IgE is utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE also has an essential role in type I hypersensitivity,[3] which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE also plays a pivotal role in responses to allergens, such as: anaphylactic drugs, bee stings, and antigen preparations used in desensitization immunotherapy. Although IgE is typically the least abundant isotype—blood serum IgE levels in a normal (non-atopic) individual are only 0.05% of the Ig concentration,[4] compared to 75% for the IgGs at 10 mg/ml, which are the isotypes responsible for most of the classical adaptive immune response—it is capable of triggering the most powerful inflammatory reactions. IgE primes the IgE-mediated allergic response by binding to Fc receptors found on the surface of mast cells and basophils. IgE may play an important role in the immune system’s recognition of cancer,[5] in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE is known to be elevated in various autoimmune disorders such as Lupus(SLE), Rheumatoid Arthritis(RA) & psoriasis, and is theorized to be of pathogenetic importance in RA and SLE by eliciting a hypersensitivity reaction.[6]

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注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。