- 首页
- 猪C反应蛋白Elisa试剂盒,BR
猪C反应蛋白Elisa试剂盒,BR
Porcine CRP Elisa Kit
(订货以英文名为准)猜你喜欢
CAS No:14808-60-7
CAS No:78081-87-5
CAS No:89640-81-3
CAS No:171919-36-1
CAS No:41317-15-1
CAS No:826-55-1
CAS No:13292-87-0
CAS No:652-03-9
CAS No:1309357-15-0
CAS No:63089-50-9
产品属性 Product Attributes
基本信息
中文名称: 猪C反应蛋白检测试剂盒英文名称: Porcine CRP ELISA Kit
别名: C-Reactive Protein; CRP; MGC88244; Pentraxin 1; PTX1MGC149895
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: CRP
样品类型: 血清/血浆/细胞培养上清
反应性: Porcine
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 78.12-5000 pg/ml
灵敏度: 36 pg/ml
特异性: 不与ApoAI,BMP1,BMP2,BMP3,BMP4,BMP5,BMP7,CRP,FGFacidic,HGF,HSP27,IL-1α,IL-1β,IL-1RA,IL-1RI,IL-2,IL-5,IL-6,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IGF1,MMP-2,MMP9,PDGF,PLA2G7,serpinE1,sIL-2R,sIL-6R,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 2.33h
Elisa信息
Swiss Prot: O19062Gene ID: 396842
检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪CRP单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的猪CRP会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗猪CRP抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的猪CRP发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的猪CRP,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的猪CRP浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中猪CRP的浓度。
背景说明: C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
产品介绍 Product Introduction
暂无数据注意事项 Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
沪公网安备31012002005909号
点击QQ联系