400-900-4166

大鼠高迁移率族蛋白B1检测试剂盒,BR

Rat HMGB1 ELISA KIT

(订货以英文名为准)
产品编号:AC16903 品牌:Acmec

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中文名称:
大鼠高迁移率族蛋白B1检测试剂盒
英文名称:
Rat HMGB1 ELISA KIT
保存条件:
2-8℃

产品属性 Product Attributes

基本信息

中文名称: 大鼠高迁移率族蛋白B1检测试剂盒
英文名称: Rat HMGB1 ELISA Kit
别名: Amphoterin; high mobility group protein B1; nonhistone chromosomal protein 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: HMGB1
样品类型: 血清/血浆/细胞培养上清
反应性: Rat
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 62.5-4,000pg/mL
灵敏度: 10pg/mL
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%

Elisa信息

Swiss Prot: P63159
Gene ID: 25459
检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠HMGB1单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品、预稀释的样本、样本稀释液和抗大鼠HMGB1检测抗体,标准品和样本中的大鼠HMGB1与检测抗体的结合物会与酶标板上的包被抗体充分结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的大鼠HMGB1,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的大鼠HMGB1浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠HMGB1的浓度。
背景说明: HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.

产品介绍 Product Introduction

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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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