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兔白介素17AElisa试剂盒,BR
Rabbit IL-17A Elisa Kit
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产品属性 Product Attributes
基本信息
中文名称: 兔白细胞介素17检测试剂盒英文名称: Rabbit IL-17 ELISA Kit
别名: Cytotoxic T-lymphocyte-associated antigen 8; IL17;; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: IL-17
样品类型: 血清/血浆/细胞培养上清
反应性: Rabbit
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 31.25-2000 pg/ml
灵敏度: 15 pg/ml
特异性: 不与兔TNF-α,IL-2,IL-4,IL-6,IFN-γ,小鼠IL-2,IL-17A,IL-4等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 2.33h
Elisa信息
Gene ID: 100339322
检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗兔IL-17A单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的兔IL-17A会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗兔IL-17A抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的兔IL-17A发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的兔IL-17A,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的兔IL-17A浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中兔IL-17A的浓度。
背景说明: Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.
产品介绍 Product Introduction
暂无数据注意事项 Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。


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