Recombin human HLA-DQB2 protein,BR

产品属性 Product Attributes

基本信息

基因名: HLA-DQB2
别名: HLA-DXB
英文名称: Recombinant human HLA-DQB2 protein
全名: major histocompatibility complex，class II，DQ beta 2
中文名称: Ⅱ类主要组织相容性复合体DQβ2
规格: 10ug ; 50ug ; 100ug ; 1mg
外观:(性状) Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.)
纯度: ≥85% by SDS-PAGE.
储存条件: Reconstituted protein solution can be stored at 4-7°C for 1-2 weeks, stored at < -20°C for 1 year.
运输条件: The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
浓度: 1mg/ml by SDS-PAGE.

产品信息

Swiss Prot： P05538
Gene ID： 3120
交叉反应： Human
分子量： 25kDa
区间范围： 1-231aa
背景说明： Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.The peptide binding cleft accommodates peptides of 10-30 residues.The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases.Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous.As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components.Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments.In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract.To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer.Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide).The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released.HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound.The MHC II molecule bound to a peptide is then transported to the cell membrane surface.In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.Primary dendritic cells (DCs) also to express HLA-DO.Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
应用： Immunology research
来源： E.coli

其它标识

纯化方法： NI-NTA affinity purification
溶剂： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
内毒素： Not measured
生物活性： N0
标签信息： With a 6×His tag at the N/C-terminus.

产品介绍 Product Introduction

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注意事项 Considerations

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