人成纤维细胞生长因子10检测试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 人成纤维细胞生长因子10检测试剂盒
英文名称： Human FGF10 ELISA Kit
别名： Fibroblast Growth Factor 10; FGF10; FGF-10; Keratinocyte growth factor 2; KGF2; KGF-2; produced by fibroblasts of urinary bladder lamina propria
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： FGF10
样品类型： 血清/血浆/细胞培养上清
反应性： Human
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 125-8000 pg/ml
灵敏度： 125-8000 pg/ml
特异性： 不与人ApoAI,BMP1,BMP2,BMP4,BMP7,HGF,HSP27,IL-1β,IL-1RA,IL-2,IL-2R,IL-5,IL-6,IL-6R,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-23,IFNγ,MMP-2,MMP-9,PDGF-AA,PDGF-BB,PDGF-AB,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Elisa信息

Swiss Prot： O15520
Gene ID： 2255
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人FGF10单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人FGF10会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人FGF10抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人FGF10发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人FGF10，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人FGF10浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人FGF10的浓度。
背景说明： The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus. It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF‑10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer.

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注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。