大鼠白细胞介素1受体2检测试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 大鼠白细胞介素1受体2检测试剂盒
英文名称： Rat IL1R2 ELISA Kit
别名： CD121b; IL-1 R beta; IL-1 RII; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta
储存条件： 2-8℃，6 months
规格： 48T ; 96T
检测目标： IL1R2
样品类型： 血清/血浆/细胞培养上清
反应性： Rat
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 12.5-800 pg/ml
灵敏度： 6.25 pg/ml
特异性： 不与大鼠IL-1R2类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Elisa信息

Swiss Prot： P43303
Gene ID： 117022
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 IL-1R2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠IL-1R会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠 IL-1R2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 IL-1R2发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 IL-1R2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 IL-1R2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 IL-1R2的浓度。
背景说明： Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

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注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。