大鼠CD93因子检测试剂盒,BR

产品属性 Product Attributes

基本信息

中文名称： 大鼠CD93因子检测试剂盒
英文名称： Rat CD93 ELISA Kit
别名： Cluster Of Differentiation 93; CD93; CD93 Molecule; C1qr1; C1qrp
储存条件： 2-8℃，6 months
规格： 48T ; 96T
检测目标： CD93
样品类型： 血清/血浆/细胞培养上清
反应性： Rat
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 31.25-2000 pg/ml
灵敏度： 15.625 pg/ml
特异性： 不与大鼠CD93类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Elisa信息

Swiss Prot： Q9ET61
Gene ID： 84398
检测原理： Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠CD93单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠CD93会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠CD93抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠CD93发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠CD93，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠CD93浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠CD93的浓度。
背景说明： CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches

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