Anti-Phospho-PRKCA(Thr638) Polyclonal Antibody,BR

产品属性 Product Attributes

基本信息

英文名称： Anti-Phospho-PRKCA(Thr638)Polyclonal Antibody
中文名称： 蛋白激酶Cα抗体
规格： 50ul ; 100ul
宿主： Rabbit
克隆类型： Polyclonal Antibody
亚型： IgG
交叉反应： Rat ; Mouse预测
交叉反应： Human;Chicken;Dog;Cow;Horse
应用： WB ; IHC-P ; IHC-F ; IF
稀释比例： WB 1:500-2000. IHC-P 1:100-500. IHC-F 1:100-500. IF 1:100-500.

抗体信息

靶标： Phospho-PRKCA(Thr638)
别名： PKC alpha (phospho T638);Phospho-PKC beta II (Thr641);PKC(Phospho-Thr641);Phospho-PKC alpha/beta II (Thr638/641);Phospho-PKC alpha/beta II (Thr638+641)
背景说明： Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis.
Swiss Prot： P17252
Gene ID： 5578
亚细胞定位： Cytoplasm Nucleus Cell membrane
研究领域： 神经学 ; 细胞生物学 ; 发育生物学 ; 信号传导 ; 表观遗传
免疫原： A synthetic peptide of human PKC alpha around the phosphorylation site of Thr638
理论分子量： 77kDa

其它标识

纯化方法： Affinity purification
储存液： 0.01M TBS (pH7.4) with 1% BSA， 0.02% Proclin300 and 50% Glycerol.
储存条件： Store at -20℃. Avoid freeze / thaw cycles.
运输条件： 冷冻运输

产品介绍 Product Introduction

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