兔抗鸡IgG(纯化),BR

产品属性 Product Attributes

基本信息

英文名称： Rabbit Anti-Chicken IgY（Purified Antibody）
中文名称： 兔抗鸡IgY（纯化抗体）
规格： 1mg ; 10mg ; 100mg
宿主： Rabbit
克隆类型： Polyclonal Antibody
亚型： IgG
交叉反应 Chicken
应用 ELISA WB
稀释比例 WB 1:1000-20000.;ELISA:1:2000-100000.

抗体信息

背景说明 In chickens，immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG，it is composed of two light and two heavy chains. Structurally，these two types of immunoglobulin differ primarily in the heavy chains，which in IgY have a molecular mass of about 65，100 atomic mass units (amu)，and are thus larger than in IgG. The light chains in IgY，with a molar mass of about 18，700 amu，are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167，000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally，IgY is partially comparable to Immunoglobulin E (IgE)，as well as to IgG. However，in contrast to IgG，IgY does not bind to Protein A，to Protein G，or to cellular Fc receptors. Furthermore，IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem，after they were able to show differences between the immunoglobulins found in chicken eggs，and immunoglobulin G. Other synonymous names are Chicken IgG，Egg Yolk IgG，and 7S-IgG.
免疫原 Chicken IgY full length protein
理论分子量 180kDa

其它标识

纯化方法： Affinity purification
储存液： Buffer: PBS with 0.03% Proclin300（PH7.2-7.4).
储存条件： Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).外观(性状) Liquid浓度 10mg/mL

产品介绍 Product Introduction

采用硫酸铵盐析与DEAE-离子交换纤维素层析，或批次吸附纯化，制成高纯度(﹥95%)和高活性的纯化IgG抗体。各种纯化抗体均为液体提供，可根据用户要求，配成不同浓度，用前最好－20℃存放，可根据使用方法采用不同浓度稀释。通常不同使用目的可分别用下列缓冲液稀释，注意完全化冻，充分混匀。用前最好低温高速离心去除冷聚合球蛋白及变性蛋白后，重新定量。
金标记：用pH9.0硼酸缓冲液稀释，1~2mg/ml标记。HRP标记：用0.01mol/L pH9.0、CB或用包被液CB稀释，6~8mg/ml标记荧光标记：用0.5mol/L pH9.5、CB(碳酸盐缓冲液)稀释，10~20 mg/ml标记。生物素记抗体：用0.1mol/L NaHCO3稀释，1~2mg/ml标记。其它方法，如免疫扩散可用0.01mol/L pH7.2 PBS稀释，ELISA包被10~20μg/ml 4℃过夜或37℃ 2h。  
备注：产品信息可能会有优化升级。请以实际标签信息为准。  

注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
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