兔抗亲合素,BR
Rabbit Anti-Avidin(Purified Antibody)
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产品属性 Product Attributes
基本信息
英文名称: Rabbit Anti-Avidin(Purified Antibody)中文名称: 兔抗Avidin(卵清亲合素)(纯化抗体)
规格: 1mg ; 10mg ; 100mg
宿主: Rabbit
亚型: IgG
交叉反应: Avidin
应用: ELISA ; WB
稀释比例: WB 1:1000-20000.;ELISA:1:2000-100000.
抗体信息
背景说明: Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds,reptiles and amphibians deposited in the whites of their eggs. In chicken egg white,avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer),each of which can bind to biotin (Vitamin B7,vitamin H) with a high degree of affinity and specificity. In its tetrameric form,avidin is estimated to be between 66–69 kDa in size.Ten percent of the molecular weight is attributed to carbohydrate content composed of four to five mannose and three N-acetylglucosamine residues. The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition.免疫原: Avidin
理论分子量: 60kDa
其它标识
纯化方法: Affinity purification储存液: Buffer: PBS with 0.03% Proclin300(PH7.2-7.4).
储存条件: Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
运输条件: 冷藏运输
外观(性状): Liquid
浓度: 10mg/mL
产品介绍 Product Introduction
本产品采用硫酸铵盐析或亲和吸附纯化,制成高纯度和高活性的纯化IgG抗体。各种纯化抗体均为液体提供,可根据用户要求,配成不同浓度,-20℃存放,可根据使用方法采用不同浓度稀释。使用前注意完全溶解,充分混匀,最好低温高速离心去除冷聚合球蛋白及变性蛋白后,重新定量后再使用。列举以下可参考的稀释缓冲液:
胶体金标记:用pH9.0硼酸缓冲液稀释,1~2mg/ml标记。
HRP标记:用0.01mol/L pH9.0、CB或用包被液CB稀释,6~8mg/ml标记。
荧光标记:用0.5mol/L pH9.5、CB(碳酸盐缓冲液)稀释,10~20 mg/ml标记。
生物素标记抗体:用0.1mol/L NaHCO3稀释,1~2mg/ml标记。
备注:产品信息可能会有优化升级。请以实际标签及说明书信息为准。
注意事项: Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。


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