Recombin human APEX2 protein,BR

产品属性 Product Attributes

基本信息

基因名: APEX2
别名: AP endonuclease XTH2；APEX nuclease 2；APEX nuclease-like 2；AP endonuclease 2
英文名称: Recombinant human APEX2 protein
全名: APEX nuclease (apurinic/apyrimidinic endonuclease) 2
中文名称: APEX核酸酶2
规格: 10ug ; 50ug ; 100ug ; 1mg
外观:(性状) Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.)
纯度: ≥85% by SDS-PAGE.
储存条件: Reconstituted protein solution can be stored at 4-7°C for 2-7 days, stored at < -20°C for 1 year.
运输条件: The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
浓度: 1mg/ml by SDS-PAGE.

产品信息

Swiss Prot： Q9UBZ4
Gene ID： 27301
交叉反应： Human Mouse (Bovine)
分子量： 39kDa
区间范围： 172-521aa
背景说明： Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities.Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially.Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents.In the nucleus functions in the PCNA-dependent BER pathway.Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes.Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
应用： Immunology research
来源： E.coli

其它标识

纯化方法： NI-NTA affinity purification
溶剂： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
内毒素： Not measured
生物活性： No activity experimental data, to be verified.
标签信息： With a 6×His tag at the N/C-terminus.

产品介绍 Product Introduction

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注意事项 Considerations

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。