Recombin human EIF2S3 protein,BR

产品属性 Product Attributes

基本信息

基因名: EIF2S3
别名: EIF2;EIF2G;EIF2gamma;MRXSBRK;eIF-2gA
英文名称: Recombinant human EIF2S3 protein
全名: eukaryotic translation initiation factor 2，subunit 3 gamma，52kDa
中文名称: 真核翻译起始因子2亚基3
规格: 10ug ; 50ug ; 100ug ; 1mg
外观:(性状) Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. (Or liquid with 5% glycerol.)
纯度: ≥85% by SDS-PAGE.
储存条件: Reconstituted protein solution can be stored at 4-7°C for 1-2 weeks, stored at < -20°C for 1 year.
运输条件: The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
浓度: 1mg/ml by SDS-PAGE.

产品信息

Swiss Prot： P41091
Gene ID： 1968
交叉反应： Human Mouse Rat (Bovine Zebrafish Orangutan Chicken Rabbit Pig)
分子量： 38kDa
区间范围： 123-472aa
背景说明： As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B. Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage.
应用： Immunology research
来源： E.coli

其它标识

纯化方法： NI-NTA affinity purification
溶剂： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
内毒素： Not measured
生物活性： N0
标签信息： With a 6×His tag at the N/C-terminus.

产品介绍 Product Introduction

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注意事项 Considerations

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