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小鼠前蛋白转化酶枯草溶菌素9检测试剂盒,BR
Mouse PCSK9 ELISA Kit
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产品属性 Product Attributes
基本信息
中文名称: 小鼠前蛋白转化酶枯草溶菌素9检测试剂盒英文名称: Mouse PCSK9 ELISA Kit
别名: FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; PC9; subtilisin/kexin-like protease PC9
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: Proprotein Convertase 9/PCSK9
样品类型: 血清/血浆/细胞培养上清
反应性: Mouse
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 156.25-10000 pg/ml
灵敏度: 78.13 pg/ml
特异性: 不与小鼠FurinLDLR,VLDLR,人PCSK1,PCSK7,PCSK9等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3h
Elisa信息
Swiss Prot: Q80W65Gene ID: 100102
检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠PCSK9单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的小鼠PCSK9会与酶标板上的包被抗体充分结合;洗板后加入辣根过氧化物酶(HRP)标记的抗小鼠PCSK9抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠PCSK9发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的小鼠PCSK9,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的小鼠PCSK9浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠PCSK9的浓度。
背景说明: Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
产品介绍 Product Introduction
暂无数据注意事项 Considerations
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。