胶原酶Ⅲ,210u/mg
CollagenaseⅢ
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胶原蛋白酶 来源于溶组织梭菌
Collagenase from Clostridium histolyticum
purified by chromatography, Type III, 2-10 FALGPA units/mg solid, ≥250 CDU/mg solid (CDU = collagen digestion units), lyophilized powder
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.
包装
Product sold on the basis of collagen digestion units (CDU).
Preparation Note
Collagenase may be dissolved in TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 °C) (AC11508)at 0.05-0.1 mg/mL concentration.
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.
type Type III
form lyophilized powder
purified by chromatography
solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble0.05-0.1 mg/mL at 37 °C
foreign activity Clostripain, may contain trace amount neutral protease storage temp. −20°C
胶原酶用途是什么?
提问时间:2021/8/9 8:42:56
胶原酶是做什么用的?哪里可以用到胶原酶?
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回复内容:
1
admin2021-08-30 09:43:10
胶原酶胶原蛋白酶用途1.主要局部外用于烧伤,尚可用于治疗溃疡、浓疮、脊椎软骨增生。 2.可用作化妆品的营养添加剂,广泛地用于香波、护发素、香皂、膏霜等化妆品中。
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胶原酶在4度冰箱放了一个月会失活吗?
提问时间:2019/7/30 16:49:39
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回复内容:
2
1329219489@qq.com2019/7/30 16:50:27
胶原酶4度存放,最好1~2周内用完;
长时间贮存宜在-20摄氏度,用前可放37度溶解,但溶解时间不能过久,防止酶活性受影响。
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胶原酶在4度冰箱放了一个月会失活吗?
提问时间:2019/6/24 14:05:13
胶原酶在4度冰箱放了一个月会失活吗?
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回复内容:
2
1329219489@qq.com2019/6/24 14:06:28
胶原酶4度存放,最好1~2周内用完;
长时间贮存宜在-20摄氏度,用前可放37度溶解,但溶解时间不能过久,防止酶活性受影响;
消化组织时间:取决于消化液浓度和组织类型,一般消化时间为2-3小时或者4度过夜。
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